pET22b-DS55
(Plasmid #187175)

• Purpose: (Empty Backbone) This modified pET22b-DS55 vector contains Sal I and Hind III sites in reverse orientations to directly clone scFv phagemid from HuScL-4 library for bacterial expression systems.
• Depositing Lab: Dinakar Salunke
• Publication: Jaiswal et al bioRxiv 2025.07.15.663531

Backbone

• Vector backbone: pET22b(+)
• Backbone manufacturer: Novagen
• Backbone size (bp): 5493
• Modifications to backbone: Nco I, Hind III, BamH I, Sal I, Xho I followed by 6 His tag
• Vector type: Bacterial Expression
• Promoter: AmpR, lac1
• Tag / Fusion Protein:
  • His tag (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We have modified pET22b(+) vector backbone MCS to directly clone scFv phagemid from HuScL-4 library.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The modification in the MCS of pET22b(+) are as follows:
Nco I, Hind III, BamH I, Sal I, Xho I followed by 6 His tag (C-ter).

Please visit https://doi.org/10.1101/2025.07.15.663531 for bioRxiv preprint.