pLKO5d.SFFV.Cre.IRES.dTomato
(Plasmid
#187192)
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PurposeLentiviral overexpression of Cre recombinase
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187192 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLKO5d.SFFV.IRES.dTomato
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre recombinase
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SpeciesEscherichia phage P1 (isolate: mod749::IS5 c1.100 mutant, nat-host: Escherichia coli)
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Entrez Genecre (a.k.a. P1_gp003)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO5d.SFFV.Cre.IRES.dTomato was a gift from Jan-Henning Klusmann (Addgene plasmid # 187192 ; http://n2t.net/addgene:187192 ; RRID:Addgene_187192) -
For your References section:
RUNX1 isoform disequilibrium promotes the development of trisomy 21-associated myeloid leukemia. Gialesaki S, Brauer-Hartmann D, Issa H, Bhayadia R, Alejo-Valle O, Verboon L, Schmell AL, Laszig S, Regenyi E, Schuschel K, Labuhn M, Ng M, Winkler R, Ihling C, Sinz A, Glass M, Huttelmaier S, Matzk S, Schmid L, Struwe FJ, Kadel SK, Reinhardt D, Yaspo ML, Heckl D, Klusmann JH. Blood. 2023 Mar 9;141(10):1105-1118. doi: 10.1182/blood.2022017619. 10.1182/blood.2022017619 PubMed 36493345