pLL5.0 ShCavin1-EGFP
(Plasmid #187253)

• Purpose: Lentiviral expression of shRNA targeting Cavin1
• Depositing Lab: Alpha Yap
• Publication: Teo et al Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi: 10.1016/j.devcel.2020.05.002. Epub 2020 Jun 1.

Backbone

• Vector backbone: pLL5.0
• Backbone size w/o insert (bp): 7569
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ShRNA for Cavin1
• gRNA/shRNA sequence: TGAGCAGCCGGACCAATAA
• Species: M. musculus (mouse)
• Insert Size (bp): 54
• Entrez Gene: Cavin1 (a.k.a. 2310075E07Rik, Cav-p60, Cavin, Ptrf)
• Promoter: U6
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hpa1 (destroyed during cloning)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: mU6 Forward Primer

Resource Information

• Supplemental Documents:
  • pLL5.0 ShCavin1-EGFP.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid made by Jessica Teo

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry