mRFP-PA-GFP-RhoAQ63L
(Plasmid #187282)

• Purpose: Express pmRFP-PAGFP-RhoA Q63L
• Depositing Lab: Alpha Yap
• Publication: Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 10.1016/j.devcel.2019.04.031. Epub 2019 May 16.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6790
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RhoA
• Species: H. sapiens (human)
• Insert Size (bp): 582
• Mutation: RhoA Q63L
• Entrez Gene: RHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
• Promoter: CMV, T7
• Tag / Fusion Protein:
  • mRFP-PAGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: EGFP, forward primer
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • mRFP-PA-GFP-RhoAQ63L.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid made by Srikanth Budnar

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry