1352wUV2 F2bbs kan GCANNNAGGk
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18752||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3890
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin and Kanamycin
Copy numberHigh Copy
Gene/Insert name3 tandem zinc fingers
SpeciesM. musculus (mouse)
Insert Size (bp)1298
MutationA Kan cassette (1090bp) is inserted between two bbsI sites in finger 2 of this construct. This cassette is here to provide a large drop out band when digested with BbsI. The digested backbone can then be used for building finger 2 libraries. Fingers one and three are anchor fingers which specify AGG and GCA, respectively.
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (not destroyed)
- 3′ cloning site BbsI (not destroyed)
- 5′ sequencing primer OMG5 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:1352wUV2 F2bbs kan GCANNNAGGk was a gift from Scot Wolfe (Addgene plasmid # 18752 ; http://n2t.net/addgene:18752 ; RRID:Addgene_18752)
For your References section:Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Meng X, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA. Nat Biotechnol. 2008 Jun . 26(6):695-701. 10.1038/nbt1398 PubMed 18500337