CMV mRuby3-Synapsin1a
(Plasmid
#187896)
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PurposeFor labeling pre-synaptic terminals
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Depositing Lab
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Depositing OrganizationCornell University
Located in the United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 187896-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 187896-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1 vector
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Backbone manufacturerClontech, Palo Alto, California
- Backbone size w/o insert (bp) 3998
- Total vector size (bp) 6839
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Modifications to backboneA plasmid to express mRuby-synapsin was generated by removing GFP from GFP-synapsin (Chi et al., 2001) using restriction sites AgeI and BglII, and substituting it in frame with mRuby obtained from pKanCMV-mRuby3-18aa-actin, which was a gift from Michael Lin (Addgene plasmid # 74255).
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSynapsin1a
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SpeciesR. norvegicus (rat)
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Insert Size (bp)2115
- Promoter CMV
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Tag
/ Fusion Protein
- mRuby3 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
F255Y and S304A mutations in Synapsin1a insert do not affect plasmid function.
DNA (Catalog # 187896-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 187896-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV mRuby3-Synapsin1a was a gift from Timothy Ryan (Addgene plasmid # 187896 ; http://n2t.net/addgene:187896 ; RRID:Addgene_187896)
Map uploaded by the depositor.
