|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22285||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6959
Vector typeMammalian Expression
Growth in Bacteria
Growth instructionsDH5a or JM 109 high efficiency
Copy numberLow Copy
Insert Size (bp)863
MutationEGFP alanine 206 changed to Lysin
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer gactcagcgctgcctcagtctg (Common Sequencing Primers)
Terms and Licenses
KDEL coding seq.=1714-1725
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMH4-SYN-EGFP-ER was a gift from Thomas Oertner (Addgene plasmid # 22285)
For your References section:Differential distribution of endoplasmic reticulum controls metabotropic signaling and plasticity at hippocampal synapses. Holbro N, Grunditz A, Oertner TG. Proc Natl Acad Sci U S A. 2009 Aug 18. ():. 10.1073/pnas.0905110106 PubMed 19706463
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.