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Addgene

pET29a-α3-E
(Plasmid #187935)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187935 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET29a
  • Backbone size w/o insert (bp) 5371
  • Total vector size (bp) 5544
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phage alpha 3 lysis protein E
  • Species
    phage
  • Insert Size (bp)
    264
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • Strep II (C terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer TAATACGACTCACTATAGG
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET29a-α3-E was a gift from Yi Ma (Addgene plasmid # 187935 ; http://n2t.net/addgene:187935 ; RRID:Addgene_187935)

  • For your References section:

    Construction and In Vitro Evaluation of a Tumor Acidic pH-Targeting Drug Delivery System Based on Escherichia coli Nissle 1917 Bacterial Ghosts. Ma Y, Liu Q, Hu A, Jiang S, Wang S, Liu R, Han K, Wang J. Bioengineering (Basel). 2022 Sep 2;9(9):433. doi: 10.3390/bioengineering9090433. 10.3390/bioengineering9090433 PubMed 36134979
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