LaminA-ECFP-SNAP
(Plasmid #187965)

• Purpose: expresses LaminA with C-terminal tags ECFP and SNAP
• Depositing Lab: Markus Wahl
• Publication: Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi: 10.1038/s42003-022-03689-y.

Backbone

• Vector backbone: pECFP-SNAP
• Backbone manufacturer: Labor Prof. Carsten Schultz, EMBL Heidelberg
• Backbone size w/o insert (bp): 6601
• Total vector size (bp): 8588
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LaminA
• Alt name: LMNA
• Alt name: LMN1
• Species: H. sapiens (human)
• Insert Size (bp): 1987
• GenBank ID: NM_001406983.1 NM_001406983.1
• Entrez Gene: LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
• Promoter: CMV
• Tag / Fusion Protein:
  • ECFP-SNAP (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-for (Microsynth)
• 3′ sequencing primer: EGFP-N-rev (Microsynth)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ordered from Addgene No55068

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry