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CyPET-Arf6
(Plasmid #18840)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18840 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 18840-D50 50 μg of DNA in Tris buffer $495
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DNA 18840-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEF4-myc-HisA
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5882
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CyPET-Arf6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1299
  • Mutation
    The sequence for the fluorescent protein CyPET was inserted into human Arf6 between amino acid residues 143 and 144, adding 6 amino acid linkers.
  • Entrez Gene
    ARF6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CyPET-Arf6 was a gift from Martin Schwartz (Addgene plasmid # 18840 ; http://n2t.net/addgene:18840 ; RRID:Addgene_18840)

  • For your References section:

    A fluorescence resonance energy transfer activation sensor for Arf6. Hall B, McLean MA, Davis K, Casanova JE, Sligar SG, Schwartz MA. Anal Biochem. 2008 Mar 15. 374(2):243-9. 10.1016/j.ab.2007.11.032 PubMed 18162163
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