|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18856||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 3000
Modifications to backbonenone
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
Growth instructionsThe Destination Vectors will only grow on ccdB resistant cells from Invitrogen, Catalog no. A10460.
Copy numberHigh Copy
/ Fusion Proteins
- CFP (N terminal on insert)
- Venus (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site att (not destroyed)
- 3′ cloning site att (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
pGWF1 s a Destination Vector. It carries the lethal ccdB gene (The ccdB gene is replaced with your gene of interest during the LR reaction). The Destination Vectors will only grow on ccdB resistant cells from Invitrogen, Catalog no. A10460.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGWF1 was a gift from Wolf Frommer (Addgene plasmid # 18856 ; http://n2t.net/addgene:18856 ; RRID:Addgene_18856)
For your References section:Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Kaper T, Lager I, Looger LL, Chermak D, Frommer WB. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. 10.1186/1754-6834-1-11 PubMed 18522753