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pGWF1
(Plasmid #18856)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18856 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSET
  • Backbone size (bp) 3000
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    The Destination Vectors will only grow on ccdB resistant cells from Invitrogen, Catalog no. A10460.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • CFP (N terminal on insert)
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site att (not destroyed)
  • 3′ cloning site att (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

pGWF1 s a Destination Vector. It carries the lethal ccdB gene (The ccdB gene is replaced with your gene of interest during the LR reaction). The Destination Vectors will only grow on ccdB resistant cells from Invitrogen, Catalog no. A10460.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWF1 was a gift from Wolf Frommer (Addgene plasmid # 18856 ; http://n2t.net/addgene:18856 ; RRID:Addgene_18856)
  • For your References section:

    Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Kaper T, Lager I, Looger LL, Chermak D, Frommer WB. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. 10.1186/1754-6834-1-11 PubMed 18522753