Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pGWF1
(Plasmid #18856)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18856 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRSET
  • Backbone size (bp) 3000
  • Modifications to backbone
    none
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    The Destination Vectors will only grow on ccdB resistant cells from Invitrogen, Catalog no. A10460.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • CFP (N terminal on insert)
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site att (not destroyed)
  • 3′ cloning site att (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pGWF1 s a Destination Vector. It carries the lethal ccdB gene (The ccdB gene is replaced with your gene of interest during the LR reaction). The Destination Vectors will only grow on ccdB resistant cells from Invitrogen, Catalog no. A10460.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGWF1 was a gift from Wolf Frommer (Addgene plasmid # 18856 ; http://n2t.net/addgene:18856 ; RRID:Addgene_18856)
  • For your References section:

    Fluorescence Resonance Energy Transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria. Kaper T, Lager I, Looger LL, Chermak D, Frommer WB. Biotechnol Biofuels. 2008 Jun 3. 1(1):11. 10.1186/1754-6834-1-11 PubMed 18522753