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N370S GBA pcDNA3.1
(Plasmid #188583)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188583 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 188583-D50 50 μg of DNA in Tris buffer $495
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DNA 188583-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA 3.1 (+)
  • Backbone manufacturer
    ThermoFisher
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GBA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1750
  • Mutation
    N370S
  • Entrez Gene
    GBA1 (a.k.a. GBA, GCB, GLUC)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (5', carrying its own ATG) (unknown if destroyed)
  • 3′ cloning site XbaI (3', carrying its own TGA) (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    N370S GBA pcDNA3.1 was a gift from Anthony Schapira (Addgene plasmid # 188583 ; http://n2t.net/addgene:188583 ; RRID:Addgene_188583)

  • For your References section:

    The GBA variant E326K is associated with alpha-synuclein aggregation and lipid droplet accumulation in human cell lines. Smith LJ, Bolsinger MM, Chau KY, Gegg ME, Schapira AHV. Hum Mol Genet. 2022 Sep 20:ddac233. doi: 10.1093/hmg/ddac233. 10.1093/hmg/ddac233 PubMed 36130205
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