|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18887||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 11000
Vector typeYeast Expression
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)1239
MutationLex-Ste11C (amino-terminal 413 amino acids)
Entrez GeneSTE11 (a.k.a. YLR362W)
- Cloning method Restriction Enzyme
- 5′ cloning site PflMI (destroyed during cloning)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer pHybLex_fwd
- 3′ sequencing primer pHybLex-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Lex-Ste11 has the 0.9kb PflMI-SalI fragment of pYBS345 (the PCR-derived Ste11 in pYBS317; see article) deleted by blunt-end ligation with Klenow. The open reading frame reads through to an unknown stop in the vector.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PYBS375 was a gift from Elaine Elion (Addgene plasmid # 18887 ; http://n2t.net/addgene:18887 ; RRID:Addgene_18887)
For your References section:Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Choi KY, Satterberg B, Lyons DM, Elion EA. Cell. 1994 Aug 12. 78(3):499-512. 10.1016/0092-8674(94)90427-8 PubMed 8062390