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Addgene

pBBR1_DVS_AF
(Plasmid #188950)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 188950 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBBR1
  • Modifications to backbone
    CIDAR MoClo cloning site, spectinomycin resistance
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Contains lacZ-alpha cassette for blue/white screening
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBBR1_DVS_AF was a gift from Eric Young (Addgene plasmid # 188950 ; http://n2t.net/addgene:188950 ; RRID:Addgene_188950)
  • For your References section:

    Systematic Part Transfer by Extending a Modular Toolkit to Diverse Bacteria. Keating KW, Young EM. ACS Synth Biol. 2023 Jun 9. doi: 10.1021/acssynbio.3c00104. 10.1021/acssynbio.3c00104 PubMed 37294017