pRubiG-T2A-AKT3(S472D)-T2A-Cre
(Plasmid #189010)

• Purpose: Retroviral vector with a ubiquitin promoter expressing GFP, human AKT3 transcript variant 1 ORF with a phosphomimetic mutation at Serine 472, and Cre recombinase, separated by T2A motifs.
• Depositing Lab: Bryan Luikart
• Publication: Retroviral vectors for expression of AKT kinases with GFP and Cre recombinase (unpublished)

Backbone

• Vector backbone: pRubiG-T2A-Cre
• Backbone manufacturer: Bryan Luikart
• Vector type: Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AKT3
• Species: H. sapiens (human)
• Mutation: Serine at 472 mutated to Aspartic Acid
• GenBank ID: NM_005465.7
• Entrez Gene: AKT3 (a.k.a. MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2)

Resource Information

• Supplemental Documents:
  • prubig-t2a-akt3s472d-t2a-cre.gb
• A portion of this plasmid was derived from a plasmid made by: ORIGENE#RC221051

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry