pRubiG-T2A-AKT1(S473A)-T2A-Cre
(Plasmid
#189012)
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PurposeRetroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF with a phosphodeficient mutation at Serine 473, and Cre recombinase, separated by T2A motifs.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 189012 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepRubiG-T2A-Cre
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Backbone manufacturerBryan Luikart
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAKT1
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SpeciesH. sapiens (human)
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MutationSerine at 473 mutated to Alanine
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GenBank IDNM_005163
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Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byORIGENE#RC220257
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRubiG-T2A-AKT1(S473A)-T2A-Cre was a gift from Bryan Luikart (Addgene plasmid # 189012 ; http://n2t.net/addgene:189012 ; RRID:Addgene_189012)