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pMKInt-hyg
(Plasmid #18953)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18953 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    phiC31 integrase
  • Species
    Streptomyces phage phiC31
  • Insert Size (bp)
    1874
  • Entrez Gene
    int (a.k.a. phiC31p51)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (destroyed during cloning)
  • 3′ cloning site EcoRV (destroyed during cloning)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pIJ8600 (Mervyn Bibb, John Innes Institute, Norwich, U.K.)
  • Terms and Licenses

Depositor Comments

Map of parent vector is uploaded

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMKInt-hyg was a gift from Michele Calos (Addgene plasmid # 18953 ; http://n2t.net/addgene:18953 ; RRID:Addgene_18953)
  • For your References section:

    Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Groth AC, Fish M, Nusse R, Calos MP. Genetics. 2004 Apr . 166(4):1775-82. 10.1534/genetics.166.4.1775 PubMed 15126397