pcDNA4-hB90
(Plasmid
#18968)
-
Depositing Lab
-
Depositing OrganizationOhio State University
Located in the United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18968 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 18968-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 18968-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepcDNA3.1+
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5500
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameBrevican C-terminus
-
Alt nameBCAN
-
Alt nameCSPG7
-
Alt nameBEHAB
-
SpeciesH. sapiens (human)
-
Insert Size (bp)2200
-
MutationHuman brevican signal peptide aa 1-23 followed by C-terminus aa 401-911. Signal peptide between BamHI-EcoRI, C-terminus between EcoRI-NotI (EcoRI site adds two extra aa)
-
Entrez GeneBCAN (a.k.a. BEHAB, CSPG7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Not I (not destroyed)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byOriginal clone MGC BC010571 purchased from Invitrogen
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 18968-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 18968-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA4-hB90 was a gift from Mariano Viapiano (Addgene plasmid # 18968 ; http://n2t.net/addgene:18968 ; RRID:Addgene_18968) -
For your References section:
The proteoglycan brevican binds to fibronectin after proteolytic cleavage and promotes glioma cell motility. Hu B, Kong LL, Matthews RT, Viapiano MS. J Biol Chem. 2008 Sep 5;283(36):24848-59. doi: 10.1074/jbc.M801433200. Epub 2008 Jul 7. 10.1074/jbc.M801433200 PubMed 18611854