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pCAG-GST-SLC-FNIP2
(Plasmid #189869)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189869 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Backbone size w/o insert (bp) 4827
  • Total vector size (bp) 9275
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SLC38A9 N-Termius and FNIP2 fusion protein
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4449
  • Mutation
    SLC38A9 residues 1-119, 10-residue GS linker
  • GenBank ID
    NM_173514 NM_020840
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCG AAA ACC TGT ATT TTC AGG GTG GTA C
  • 3′ sequencing primer GCA GGG TGG GGG CCA TGG TAC CGG AGC CGC TCC CTG ATC CGG AGC CAG ACC CGG ATG TGT TTT TTC CAT ACC C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-GST-SLC-FNIP2 was a gift from James Hurley (Addgene plasmid # 189869 ; http://n2t.net/addgene:189869 ; RRID:Addgene_189869)

  • For your References section:

    Structural basis for FLCN RagC GAP activation in MiT-TFE substrate-selective mTORC1 regulation. Jansen RM, Peruzzo R, Fromm SA, Yokom AL, Zoncu R, Hurley JH. Sci Adv. 2022 Sep 16;8(37):eadd2926. doi: 10.1126/sciadv.add2926. Epub 2022 Sep 14. 10.1126/sciadv.add2926 PubMed 36103527
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