pINDUCER20_mGFP
(Plasmid #190027)

• Purpose: (Empty Backbone) Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mGFP
• Depositing Lab: Andrew Tutt
• Publication: Tutt Lab Plasmids (2022) (unpublished)

Backbone

• Vector backbone: pINDUCER20
• Backbone manufacturer: Stephen Elledge (Addgene #44012)
• Backbone size (bp): 12546
• Modifications to backbone: XhoI flanking cassette containing in frame mGFP tag sequence inserted at vector digested XhoI site upon linearisation, directionality post-ligation confirmed by clonal sequencing
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): ccdB Survival
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: LNCX

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

C terminal mGFP on backbone