pBeast-pAhpC-RBS- sfGFP
(Plasmid
#190051)
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PurposeGFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter
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Depositing Lab
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Depositing OrganizationCNRS UMR 5048, INSERM U1054, Centre de Biochimie Structurale
Located in France -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190051 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 190051-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 190051-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepBEST-OR2-OR1-Pr-UTR1-deGFP-T500
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Modifications to backboneTwo 40bp spacers were added to the each end of the insert to facilitate Gibson assembly cloning into more complex constructs. Upstream and downstream terminators (B0014 and B0015, respectively) we also added, each flanked by two 40bp spacers. The OR2-OR1-Pr promoter and UTR were replaced by the OxyR-activated pAhpC and its native RBS, and deGFP was replaced by sfGFP.
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Vector typeSynthetic Biology ; Cell-Free Protein Synthesis
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesfGFP
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SpeciesSynthetic
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Insert Size (bp)720
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 190051-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 190051-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBeast-pAhpC-RBS- sfGFP was a gift from Jerome Bonnet (Addgene plasmid # 190051 ; http://n2t.net/addgene:190051 ; RRID:Addgene_190051) -
For your References section:
PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H(2)O(2) as Signal Integrator. Soudier P, Zuniga A, Duigou T, Voyvodic PL, Bazi-Kabbaj K, Kushwaha M, Vendrell JA, Solassol J, Bonnet J, Faulon JL. ACS Synth Biol. 2022 Aug 19;11(8):2578-2588. doi: 10.1021/acssynbio.2c00138. Epub 2022 Aug 1. 10.1021/acssynbio.2c00138 PubMed 35913043
