pBeast-pAhpC-RBS- LacZ
(Plasmid
#190060)
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PurposeLacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBEST-OR2-OR1-Pr-UTR1-deGFP-T500
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Modifications to backboneTwo 40bp spacers were added to the each end of the insert to facilitate Gibson assembly cloning into more complex constructs. Upstream and downstream terminators (B0014 and B0015, respectively) we also added, each flanked by two 40bp spacers. The OR2-OR1-Pr promoter and UTR were replaced by the OxyR-activated pAhpC and its native RBS, and deGFP was replaced by lacZ.
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Vector typeSynthetic Biology ; Cell-Free Protein Synthesis
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelacZ
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SpeciesEscherichia coli
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Insert Size (bp)3075
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBeast-pAhpC-RBS- LacZ was a gift from Jerome Bonnet (Addgene plasmid # 190060 ; http://n2t.net/addgene:190060 ; RRID:Addgene_190060) -
For your References section:
PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H(2)O(2) as Signal Integrator. Soudier P, Zuniga A, Duigou T, Voyvodic PL, Bazi-Kabbaj K, Kushwaha M, Vendrell JA, Solassol J, Bonnet J, Faulon JL. ACS Synth Biol. 2022 Aug 19;11(8):2578-2588. doi: 10.1021/acssynbio.2c00138. Epub 2022 Aug 1. 10.1021/acssynbio.2c00138 PubMed 35913043