PeroxiHUB: A Modular Cell-Free Biosensing Platform Using H(2)O(2) as Signal Integrator.
Soudier P, Zuniga A, Duigou T, Voyvodic PL, Bazi-Kabbaj K, Kushwaha M, Vendrell JA, Solassol J, Bonnet J, Faulon JL
ACS Synth Biol. 2022 Aug 19;11(8):2578-2588. doi: 10.1021/acssynbio.2c00138. Epub 2022 Aug 1. PubMed Article
Soudier P, Zuniga A, Duigou T, Voyvodic PL, Bazi-Kabbaj K, Kushwaha M, Vendrell JA, Solassol J, Bonnet J, Faulon JL
ACS Synth Biol. 2022 Aug 19;11(8):2578-2588. doi: 10.1021/acssynbio.2c00138. Epub 2022 Aug 1. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 190051 | pBeast-pAhpC-RBS- sfGFP | GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter |
| 190052 | pBeast-pOxyS-RBS- sfGFP | GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pOxyS promoter |
| 190053 | pBeast-pKatG-RBS- sfGFP | GFP containing reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pKatG promoter |
| 190054 | pBeast-pYjjZ-RBS- sfGFP | GFP containing reporter plasmid expressing it under the control of the reportedely OxyR/H2O2 inducible pYjjZ promoter |
| 190057 | pBeast-pT7-soxA | Express the bacterial monomeric sarcosine oxidase enzyme under the control of the strong constitutif phage promoter T7 |
| 190058 | pBeast-pT7-codA | Express the bacterial choline oxidase enzyme under the control of the strong constitutif phage promoter T7 |
| 190059 | pBeast-pT7-lox | Express the bacterial lactate oxidase enzyme under the control of the strong constitutif phage promoter T7 |
| 190060 | pBeast-pAhpC-RBS- LacZ | LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter |