pMEXneo-TrkC-TrkB-hybrid
(Plasmid
#190205)
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PurposeTo express a Human TrkC receptor that binds only BDNF. The second IgG like domain which is the main binding domain comes from the TrkB receptor
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Depositing Lab
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Depositing OrganizationUniversity of Miami
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190205 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 190205-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 190205-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMEXneo
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Backbone manufacturerForough R, et al. J Biol Chem. 1993 Feb 5;268(4):2960-8. PMID: 7679105
- Backbone size w/o insert (bp) 6392
- Total vector size (bp) 8949
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTrkC
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Alt nameNTRK3 or gp145(trkC)
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SpeciesH. sapiens (human)
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Insert Size (bp)2557
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MutationThe second IgG like domain and the extracellular juxtamembrane domain are replaced with the human TrkB sequences
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GenBank IDAAA75374.1, AAB33111.1, AAB33112.1, NP_001007157.1, NP_001012338.1, NP_001230030.1, NP_002521.2, Q16288.2,
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Entrez GeneNTRK3 (a.k.a. GP145-TrkC, TRKC, gp145(trkC))
- Promoter Moloney mouse sarcoma virus right LTR
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SbfI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer EBV-rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 190205-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 190205-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMEXneo-TrkC-TrkB-hybrid was a gift from Pantelis Tsoulfas (Addgene plasmid # 190205 ; http://n2t.net/addgene:190205 ; RRID:Addgene_190205) -
For your References section:
High resolution mapping of the binding site of TrkA for nerve growth factor and TrkC for neurotrophin-3 on the second immunoglobulin-like domain of the Trk receptors. Urfer R, Tsoulfas P, O'Connell L, Hongo JA, Zhao W, Presta LG. J Biol Chem. 1998 Mar 6;273(10):5829-40. doi: 10.1074/jbc.273.10.5829. 10.1074/jbc.273.10.5829 PubMed 9488719
Map uploaded by the depositor.
