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ADH1pro_LEU3_SIR4
(Plasmid #190582)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190582 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    unknown
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LEU3
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2658
  • Entrez Gene
    LEU3 (a.k.a. YLR451W)
  • Promoter ADH1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ADH1pro_LEU3_SIR4 was a gift from Rob Mitra (Addgene plasmid # 190582 ; http://n2t.net/addgene:190582 ; RRID:Addgene_190582)

  • For your References section:

    Zinc cluster transcription factors frequently activate target genes using a non-canonical half-site binding mode. Recio PS, Mitra NJ, Shively CA, Song D, Jaramillo G, Lewis KS, Chen X, Mitra RD. Nucleic Acids Res. 2023 Jun 9;51(10):5006-5021. doi: 10.1093/nar/gkad320. 10.1093/nar/gkad320 PubMed 37125648
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