TEF2pro_mCherry_YFP
(Plasmid
#190588)
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PurposeDual reporter backbone plasmid for CCRA experiment- LEU2 Marker
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepRS414
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TEF2pro_mCherry_YFP was a gift from Rob Mitra (Addgene plasmid # 190588 ; http://n2t.net/addgene:190588 ; RRID:Addgene_190588) -
For your References section:
Zinc cluster transcription factors frequently activate target genes using a non-canonical half-site binding mode. Recio PS, Mitra NJ, Shively CA, Song D, Jaramillo G, Lewis KS, Chen X, Mitra RD. Nucleic Acids Res. 2023 Jun 9;51(10):5006-5021. doi: 10.1093/nar/gkad320. 10.1093/nar/gkad320 PubMed 37125648