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Addgene

pCAG-HA-Sema7A
(Plasmid #190644)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190644 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sema7A
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Sema7a (a.k.a. 2900057C09Rik, CDw108, H-Sema K1, H-Sema-L, M-Sema-L, Semal, sema K1, sema L)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CAG-FW
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HA-Sema7A was a gift from Andrew Newman & Victor Tarabykin (Addgene plasmid # 190644 ; http://n2t.net/addgene:190644 ; RRID:Addgene_190644)

  • For your References section:

    Semaphorin heterodimerization in cis regulates membrane targeting and neocortical wiring. Bessa P, Newman AG, Yan K, Schaub T, Dannenberg R, Lajko D, Eilenberger J, Brunet T, Textoris-Taube K, Kemmler E, Deng P, Banerjee P, Ravindran E, Preissner R, Rosario M, Tarabykin V. Nat Commun. 2024 Aug 16;15(1):7059. doi: 10.1038/s41467-024-51009-1. 10.1038/s41467-024-51009-1 PubMed 39152101
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