pCAG-sp-myc-hSema4D-Q497P
(Plasmid
#190655)
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PurposeExpresses Human Sema4D with N terminal myc after signal peptide containing 497P mutation
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190655 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSema4D
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SpeciesH. sapiens (human)
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Mutation497P
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Entrez GeneSEMA4D (a.k.a. A8, BB18, C9orf164, CD100, COLL4, GR3, M-sema-G, SEMAJ, coll-4)
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Tag
/ Fusion Protein
- myc (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CAG-FW (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-sp-myc-hSema4D-Q497P was a gift from Andrew Newman & Victor Tarabykin (Addgene plasmid # 190655 ; http://n2t.net/addgene:190655 ; RRID:Addgene_190655) -
For your References section:
Semaphorin heterodimerization in cis regulates membrane targeting and neocortical wiring. Bessa P, Newman AG, Yan K, Schaub T, Dannenberg R, Lajko D, Eilenberger J, Brunet T, Textoris-Taube K, Kemmler E, Deng P, Banerjee P, Ravindran E, Preissner R, Rosario M, Tarabykin V. Nat Commun. 2024 Aug 16;15(1):7059. doi: 10.1038/s41467-024-51009-1. 10.1038/s41467-024-51009-1 PubMed 39152101