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Addgene

DR5::TMK1C-3FLAG
(Plasmid #190765)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190765 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pW1211
  • Backbone size w/o insert (bp) 12000
  • Total vector size (bp) 15000
  • Vector type
    Plant Expression ; Agrobacterium Binary
  • Selectable markers
    kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    28C in Agrobacterium tumefaciens
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TMK1 Transmembrane Kinase 1 Cytoplasmic Domain (amino acids 505-942)
  • Alt name
    At1g66150
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1496
  • Mutation
    truncated protein (amino acids 505-942) containing the cytoplasmic kinase domain
  • GenBank ID
    NM_105286
  • Entrez Gene
    TMK1 (a.k.a. AT1G66150, F15E12.4, F15E12_4, transmembrane kinase 1)
  • Promoter Auxin-dependent DR5 containing 9 reverse TGTCTC sites and -46 35S promoter (Addgene 61628) GeneBank ID KJ081243.1
  • Tag / Fusion Protein
    • 3FLAG (C terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer ggatccatggtttccaaggg
  • 3′ sequencing primer CATCGTCCATCTACTGAAGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DR5::TMK1C-3FLAG was a gift from Michael Bevan (Addgene plasmid # 190765)
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