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Addgene

pNS1D-sgRNA9-SpR
(Plasmid #191092)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191092 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCas34
  • Backbone size (bp) 6428
  • Modifications to backbone
    A fragment containing two BbsI sites for Golden Gate assembly of the 20 bp crRNA oligonucleotide was inserted upstream of the gRNA scaffold sequence by Gibson assembly. The kanamycin resistance cassette was replaced with a spectinomycin resistance cassette. A BbsI site in the plasmid backbone was removed.
  • Vector type
    Bacterial Expression, CRISPR
  • Promoter tet

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNS1D-sgRNA9-SpR was a gift from Anne Ruffing (Addgene plasmid # 191092 ; http://n2t.net/addgene:191092 ; RRID:Addgene_191092)

  • For your References section:

    High-Density Guide RNA Tiling and Machine Learning for Designing CRISPR Interference in Synechococcus sp. PCC 7002. Dallo T, Krishnakumar R, Kolker SD, Ruffing AM. ACS Synth Biol. 2023 Apr 21;12(4):1175-1186. doi: 10.1021/acssynbio.2c00653. Epub 2023 Mar 9. 10.1021/acssynbio.2c00653 PubMed 36893454
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