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Addgene

p3xFLAG-TMSB10
(Plasmid #191133)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191133 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    p3xFLAG-CMV10
  • Backbone manufacturer
    Sigma-Aldrich
  • Backbone size w/o insert (bp) 6279
  • Total vector size (bp) 6414
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Thymosin beta 10
  • Alt name
    TMSB10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    135
  • GenBank ID
    NM_025284.5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer ACAAAGCTTATGGCAGACAAGCCGGACATG
  • 3′ sequencing primer ACAAGATCTTTAGGAGATTTCACTCCTCT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-TMSB10 was a gift from Ken-ichirou Morohashi (Addgene plasmid # 191133 ; http://n2t.net/addgene:191133 ; RRID:Addgene_191133)

  • For your References section:

    Tmsb10 triggers fetal Leydig differentiation by suppressing the RAS/ERK pathway. Inoue M, Baba T, Takahashi F, Terao M, Yanai S, Shima Y, Saito D, Sugihara K, Miura T, Takada S, Suyama M, Ohkawa Y, Morohashi KI. Commun Biol. 2022 Sep 15;5(1):974. doi: 10.1038/s42003-022-03941-5. 10.1038/s42003-022-03941-5 PubMed 36109592
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