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Addgene

pCMV-HA-KRAS
(Plasmid #191134)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191134 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV-HA
  • Backbone size w/o insert (bp) 3764
  • Total vector size (bp) 4331
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Kirsten rat sarcoma viral oncogene
  • Alt name
    Kras
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    567
  • GenBank ID
    NM_021284.7
  • Entrez Gene
    Kras (a.k.a. K-Ras, K-Ras 2, K-ras, Ki-ras, Kras-2, Kras2, c-K-ras, c-Ki-ras, p21B, ras)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer ACAGTCGACCATGACTGAGTATAAACTTGT
  • 3′ sequencing primer ACAGCGGCCGCTCACATAACTGTACACCTTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-HA-KRAS was a gift from Ken-ichirou Morohashi (Addgene plasmid # 191134 ; http://n2t.net/addgene:191134 ; RRID:Addgene_191134)

  • For your References section:

    Tmsb10 triggers fetal Leydig differentiation by suppressing the RAS/ERK pathway. Inoue M, Baba T, Takahashi F, Terao M, Yanai S, Shima Y, Saito D, Sugihara K, Miura T, Takada S, Suyama M, Ohkawa Y, Morohashi KI. Commun Biol. 2022 Sep 15;5(1):974. doi: 10.1038/s42003-022-03941-5. 10.1038/s42003-022-03941-5 PubMed 36109592
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