pLD-puro-Cc-IFIT5_K197R-VA
(Plasmid
#191223)
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PurposeLentiviral expression of human IFIT5_K197R in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191223 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLD-puro-CcVA (Addgene plasmid #24588)
- Backbone size w/o insert (bp) 7737
- Total vector size (bp) 9183
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameinterferon-induced protein with tetratricopeptide repeats 5
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Alt nameIFIT5
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SpeciesH. sapiens (human)
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Insert Size (bp)1446
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Mutationchanged Lysine 197 to Arginine (K197R)
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GenBank IDNM_012420.3
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Entrez GeneIFIT5 (a.k.a. ISG58, P58, RI58)
- Promoter CMV
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Tag
/ Fusion Protein
- VA tag (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hPGK_R [5'-ccttggaaaaggcgcaacc-3'] (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLD-puro-Cc-IFIT5_K197R-VA was a gift from Mohan Babu (Addgene plasmid # 191223 ; http://n2t.net/addgene:191223 ; RRID:Addgene_191223)