P74-26 Tail Tube Protein (gp93) in pSMT3
(Plasmid #191261)

• Purpose: Expresses P74-26 gp93 with a His-SUMO tag in bacterial cells
• Depositing Lab: Brian Kelch
• Publication: Agnello et al J Biol Chem. 2023 Feb 13:103021. doi: 10.1016/j.jbc.2023.103021.

Backbone

• Vector backbone: pSMT3
• Backbone size w/o insert (bp): 5599
• Total vector size (bp): 6646
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: We recommend expressing the plasmid in ArcticExpress (DE3) cells.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: P74-26 gp93
• Alt name: P74-26 Tail Tube Protein
• Alt name: P74-26 TTP
• Species: Thermus Virus P74-26
• Insert Size (bp): 1044
• GenBank ID: EU100884.1
• Promoter: T7
• Tag / Fusion Protein:
  • His-SUMO (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: GATCGGATCCATGCGTGGTGTGGACACC
• 3′ sequencing primer: GATCCTCGAGTTAGAATTTCGCAACGTGGGTCAC

Resource Information

• Supplemental Documents:
  • P7426_TTP_pSMT3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry