pXD70Tet(DadRmt2)-DadR(ΔTM)
(Plasmid
#191638)
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PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔTM) complementation
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191638 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepXD69m1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsE. lenta: Tetracycline 20 μg/mL
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDadR
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SpeciesEggerthella lenta
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MutationΔTM
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pXD70Tet(DadRmt2)-DadR(ΔTM) was a gift from Emily Balskus (Addgene plasmid # 191638 ; http://n2t.net/addgene:191638 ; RRID:Addgene_191638) -
For your References section:
Genetic manipulation of the human gut bacterium Eggerthella lenta reveals a widespread family of transcriptional regulators. Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, Turnbaugh PJ, Balskus EP. Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3. 10.1038/s41467-022-33576-3 PubMed 36494336
