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Genetic manipulation of the human gut bacterium Eggerthella lenta reveals a widespread family of transcriptional regulators.
Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, Turnbaugh PJ, Balskus EP
Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
191246pXD69m1-TetWE. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lenta
191247pXD69m2-AphAE. coli - Eggerthella lenta shuttle plasmid with kanamycin resistance in E. lenta
191248pXD68Kan2-AphARSF1010-based broad host-range plasmid engineered from pAM5409, kanamycin resistance
191617pXD70LacZ1-promoterless-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZ
191618pXD70LacZ2-Pdadh-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ
191619pXD70LacZ3-Pcsd-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd promoter-lacZ, E. lenta constitutive promoter
191620pXD70LacZ4-PdegV-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), PdegV promoter-lacZ, E. lenta constitutive promoter
191621pXD70LacZ6-LacI-Ptac(LacO)-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X
191622pXD70LacZ7-DadR/DadS-Pdadh-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), Pdadh dopamine dehydroxylase promoter-lacZ with transcriptional regulators DadR/DadS
191623pXD70LacZ8-CadR-Pcadh-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ with transcriptional regulator CadR
191624pXD70LacZ8.1-Pcadh-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin dehydroxylase promoter-lacZ
191625pXD70LacZ9-Phcdh-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ
191627pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ
191628pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZ
191629pXD70LacZ9.1-HcdR-Phcdh-LacZE. coli - Eggerthella lenta shuttle plasmid (KanR), Phcdh hydrocaffeic acid dehydroxylase promoter-lacZ with transcriptional regulator HcdR
191630pXD70DA7-DadR/DadS-Pdadh-DadhABC(A2)E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulators DadR/DadS
191631pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS
191632pXD70DA9-Pct5-DadhABC(A2)E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain
191633pXD71Cas10.0-Pct5.1-crRNA(AarI)E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer), entry plasmid for crRNA cloning
191634pXD71Cas10.1-Pct5.1-crRNA(dadR)E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer)
191635pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion
191636pXD70Tet(DadR)-DadRE. coli - Eggerthella lenta shuttle plasmid (TetR), dadR complementation
191637pXD70Tet(DadRmt1)-DadR(ΔDBD)E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔDBD) complementation
191638pXD70Tet(DadRmt2)-DadR(ΔTM)E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔTM) complementation
191639pXD70Tet(DadRmt4)-DadR(linker)E. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(linker region shuffled) complementation
191640pXD70Tet(DadR-NFLAG)-N-3xFLAG-DadRE. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR complementation
191641pXD70Tet(DadRmt5)-N-3xFLAG-DadR(ΔDBD)E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔDBD) complementation
191642pXD70Tet(DadRmt6)-N-3xFLAG-DadR(ΔTM)E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(ΔTM) complementation
191643pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementation
191644pXD70Tet(DadH)-Pdadh-DadhABC(A2)E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain
191645pXD70Tet(DadHR)-DadR-Pdadh-DadhABC(A2)E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain with transcriptional regulator DadR
191646pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletion
191647pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion
191648pXD70Tet(HcdR)-HcdRE. coli - Eggerthella lenta shuttle plasmid (TetR), hcdR complementation
191649pXD70Tet(CadR)-CadRE. coli - Eggerthella lenta shuttle plasmid (TetR), cadR complementation
191650pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion
191651pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4
192273pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFPE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

Antibodies from Article