PurposeExpresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||192260||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7307
- Total vector size (bp) 7711
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
SpeciesH. sapiens (human)
Insert Size (bp)404
Entrez GenePARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
- Promoter CMV
/ Fusion Protein
- mCherry (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BlpI (not destroyed)
- 3′ cloning site EcoRV (not destroyed)
- 5′ sequencing primer CMV
- 3′ sequencing primer SV40 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byBackbone gifted from Dr. G. Timinszky (Hungarian Academy of Sciences)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMV-PARP1_D770A-mCherry was a gift from Michael Cohen (Addgene plasmid # 192260 ; http://n2t.net/addgene:192260 ; RRID:Addgene_192260)
For your References section:Allosteric regulation of DNA binding and target residence time drive the cytotoxicity of phthalazinone-based PARP-1 inhibitors. Arnold MR, Langelier MF, Gartrell J, Kirby IT, Sanderson DJ, Bejan DS, Sileikyte J, Sundalam SK, Nagarajan S, Marimuthu P, Duell AK, Shelat AA, Pascal JM, Cohen MS. Cell Chem Biol. 2022 Dec 15;29(12):1694-1708.e10. doi: 10.1016/j.chembiol.2022.11.006. Epub 2022 Dec 8. 10.1016/j.chembiol.2022.11.006 PubMed 36493759