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pCMV-PARP1_D770A-mCherry
(Plasmid #192260)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 192260 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-PARP1-mCherry
  • Backbone size w/o insert (bp) 7307
  • Total vector size (bp) 7711
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PARP1_D770A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    404
  • Mutation
    D770A
  • Entrez Gene
    PARP1 (a.k.a. ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BlpI (not destroyed)
  • 3′ cloning site EcoRV (not destroyed)
  • 5′ sequencing primer CMV
  • 3′ sequencing primer SV40
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Backbone gifted from Dr. G. Timinszky (Hungarian Academy of Sciences)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-PARP1_D770A-mCherry was a gift from Michael Cohen (Addgene plasmid # 192260 ; http://n2t.net/addgene:192260 ; RRID:Addgene_192260)
  • For your References section:

    Allosteric regulation of DNA binding and target residence time drive the cytotoxicity of phthalazinone-based PARP-1 inhibitors. Arnold MR, Langelier MF, Gartrell J, Kirby IT, Sanderson DJ, Bejan DS, Sileikyte J, Sundalam SK, Nagarajan S, Marimuthu P, Duell AK, Shelat AA, Pascal JM, Cohen MS. Cell Chem Biol. 2022 Dec 15;29(12):1694-1708.e10. doi: 10.1016/j.chembiol.2022.11.006. Epub 2022 Dec 8. 10.1016/j.chembiol.2022.11.006 PubMed 36493759