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pGL2Basic-EcadK1
(Plasmid #19290)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19290 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 19290-D50 50 μg of DNA in Tris buffer $495
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DNA 19290-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pGL2Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5568
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E-cadherin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    233
  • Mutation
    E-cad promoter sequences from -108 to +125

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer luc-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Endogenous ATG of Ecad destroyed; "C" deleted 5 bp 5' of ATG (the destroyed one)

33 bp linker between Ecad promoter and luciferace gene.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2Basic-EcadK1 was a gift from Eric Fearon (Addgene plasmid # 19290 ; http://n2t.net/addgene:19290 ; RRID:Addgene_19290)

  • For your References section:

    Extinction of E-cadherin expression in breast cancer via a dominant repression pathway acting on proximal promoter elements. Hajra KM, Ji X, Fearon ER. Oncogene. 1999 Dec 2. 18(51):7274-9. 10.1038/sj.onc.1203336 PubMed 10602481
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