Zic4-3505:GFP
(Plasmid
#193001)
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PurposePlasmid to generate a transgenic Hydra Zic4 reporter line
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Depositing Lab
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Depositing OrganizationUniversity of Geneva
Located in Switzerland -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193001 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 193001-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 193001-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneHyActin-RFP::HyWnt3FL-EGFP
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Vector typeHydra transgenesis vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHydra Zic4 promoter
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SpeciesHydra
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Insert Size (bp)3505
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit for https://www.biorxiv.org/content/10.1101/2021.12.22.473838v1 bioRxiv preprint.
DNA (Catalog # 193001-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 193001-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Zic4-3505:GFP was a gift from Brigitte Galliot (Addgene plasmid # 193001 ; http://n2t.net/addgene:193001 ; RRID:Addgene_193001) -
For your References section:
The transcription factor Zic4 acts as a transdifferentiation switch. Vogg MC, Ferenc J, Buzgariu WC, Perruchoud C , Papasaikas P, Sanchez PGL, Nuninger C , Delucinge-Vivier C , Rampon C , Beccari L, Vriz S , Vincent S , Galliot B , Tsiairis CD. bioRxiv 2021.12.22.47383 10.1101/2021.12.22.473838
