pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)
(Plasmid
#193048)
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PurposeHeatshock expressed Cas9
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193048 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC19
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Backbone manufacturerNew England Biolabs
- Backbone size w/o insert (bp) 2686
- Total vector size (bp) 2686
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Vector typeWorm Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
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Alt nameHeat shock inducible Cas9
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SpeciesC. elegans (nematode)
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Insert Size (bp)5168
- Promoter hsp16.41p
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer caggaaacagctatgaccatg (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byhsp16.41 was derived from pMA122 acquired from Addgene, Cas9(dpiRNA)::tbb-2 was derived from pCFJ150-Cas9 acquired from Addgene
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.10.30.514301v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR) was a gift from Patrick Phillips (Addgene plasmid # 193048 ; http://n2t.net/addgene:193048 ; RRID:Addgene_193048) -
For your References section:
High-throughput library transgenesis in Caenorhabditis elegans via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS). Stevenson ZC, Moerdyk-Schauwecker MJ, Banse SA, Patel DS, Lu H, Phillips PC. Elife. 2023 Jul 4;12:RP84831. doi: 10.7554/eLife.84831. 10.7554/eLife.84831 PubMed 37401921
Map uploaded by the depositor.
