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(Plasmid #193349)


Item Catalog # Description Quantity Price (USD)
Plasmid 193349 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6247
  • Total vector size (bp) 10414
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    Sox2-17 is engineered highly cooperative chimeric transcription factor, where the following elements of Sox2 were swapped with Sox17: 43-47, 61, 65-86 and CTD
  • Entrez Gene
    Klf4 (a.k.a. EZF, Gklf, Zie)
  • Entrez Gene
    Sox2 (a.k.a. Sox-2, lcc, ysb)
  • Promoter TetO mini CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (additional NotI site is present in Sox2-17 sequence) (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer gagacgccatccacgctgt
  • 3′ sequencing primer AGGAAGGTCCGCTGGATTGA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc was a gift from Hans Schöler (Addgene plasmid # 193349 ; ; RRID:Addgene_193349)
  • For your References section:

    Enhancing Sox/Oct cooperativity induces higher-grade developmental reset. MacCarthy CM, Malik V, Wu G, Velychko T, Keshet G, Jauch R, Cojocaru V, Schöler HR, Velychko S. bioRxiv 2022.09.23.509242 10.1101/2022.09.23.509242