p3E ubb polyA
(Plasmid
#195977)
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PurposeGateway p3E vector
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonep3E
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Vector typeOther
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameubb:polyA
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SpeciesD. rerio (zebrafish)
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Entrez Geneubb (a.k.a. Ubi-p63E, im:6892314, si:ch211-202a12.3, ubc, zUBC, zgc:172187)
Cloning Information
- Cloning method TOPO Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.12.13.520107v1.article-info for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3E ubb polyA was a gift from Alexa Burger & Christian Mosimann (Addgene plasmid # 195977 ; http://n2t.net/addgene:195977 ; RRID:Addgene_195977) -
For your References section:
Next-generation plasmids for transgenesis in zebrafish and beyond. Kemmler CL, Moran HR, Murray BF, Scoresby A, Klem JR, Eckert RL, Lepovsky E, Bertho S, Nieuwenhuize S, Burger S, D'Agati G, Betz C, Puller AC, Felker A, Ditrychova K, Botschi S, Affolter M, Rohner N, Lovely CB, Kwan KM, Burger A, Mosimann C. Development. 2023 Mar 28:dev.201531. doi: 10.1242/dev.201531. 10.1242/dev.201531 PubMed 36975217
Map uploaded by the depositor.
