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pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP
(Plasmid #196235)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196235 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    lentiviral
  • Backbone manufacturer
    Vectorbuilder
  • Backbone size w/o insert (bp) 8939
  • Total vector size (bp) 10283
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C-terminal EGFP tagged Af1521(K35E/Y145R)
  • Alt name
    Engineered macrodomain with (K35E/Y145R) mutation
  • Species
    Archaeoglobus fulgidus
  • Insert Size (bp)
    1347
  • Mutation
    K35E and Y145R
  • Promoter EF1A
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.12.29.573650 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP was a gift from Robert Sobol (Addgene plasmid # 196235 ; http://n2t.net/addgene:196235 ; RRID:Addgene_196235)

  • For your References section:

    Overexpression of the WWE domain of RNF146 modulates poly-(ADP)-ribose dynamics at sites of DNA damage. Al-Rahahleh RQ, Roos WP, Saville KM, Andrews JF, Wu Z, Koczor CA, Prakash A, Sobol RW. DNA Repair (Amst). 2025 Jun;150:103845. doi: 10.1016/j.dnarep.2025.103845. Epub 2025 May 21. 10.1016/j.dnarep.2025.103845 PubMed 40403420
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