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pGEX-4T-3-GST-Tev-RNF146(100-182)-myc
(Plasmid #196240)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196240 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pGEX-4T-3
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 4268
  • Total vector size (bp) 5315
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    N-terminal GST fusion of RNF146(100-182) with a TEV protease site between GST and RNF146(100-182) encoding a C-terminal myc tag
  • Alt name
    WWE domain of RNF146 (100-182)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1044
  • Tags / Fusion Proteins
    • myc tag (C terminal on insert)
    • GST tag (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Vector was custom-designed in the Sobol lab and synthesized by Genscript.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.12.29.573650 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-3-GST-Tev-RNF146(100-182)-myc was a gift from Robert Sobol (Addgene plasmid # 196240 ; http://n2t.net/addgene:196240 ; RRID:Addgene_196240)

  • For your References section:

    Overexpression of the WWE domain of RNF146 modulates poly-(ADP)-ribose dynamics at sites of DNA damage. Al-Rahahleh RQ, Roos WP, Saville KM, Andrews JF, Wu Z, Koczor CA, Prakash A, Sobol RW. DNA Repair (Amst). 2025 Jun;150:103845. doi: 10.1016/j.dnarep.2025.103845. Epub 2025 May 21. 10.1016/j.dnarep.2025.103845 PubMed 40403420
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