TK310
(Bacterial strain
#196340)
-
PurposeBacterial strain lacking adenlyate cyclase (cyaA, needed for cAMP synthesis) and phosphodiesterase (cpdA, which degrades cAMP); unable to control its intracellular cAMP levels
-
Depositing Labs
-
Sequence Information
-
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 196340 | Bacteria in agar stab | 1 | $89 | |
Backbone
-
Vector backbonen/a
-
Vector typeThis is a strain, not a plasmid
Growth in Bacteria
-
Bacterial Resistance(s)None
-
Growth Temperature37°C
-
Growth Strain(s)TK310
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameΔcyaA ΔcpdA ΔlacY
-
Speciesn/a
Cloning Information
- Cloning method Unknown
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Primers:
oSK285, cpdA-fwd: GAGTGGGCATAAATGAAGCG
oSK286, cpdA-rev: CACTGCGGGCAGCATAAA
oSK287, lacY-fwd: CCGGTCGCTACCATTACCAG
oSK288, lacY-rev: CTTTCGGTCATTGGCATGTTC
oSK289, CyaA-fwd: GCGCATCTTTCTTTACGGTC
oSK290, CyaA-rev: GCTGCACCAGGTATGGCT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
TK310 was a gift from Terence Hwa & Thomas Kuhlman (Addgene plasmid # 196340) -
For your References section:
Combinatorial transcriptional control of the lactose operon of Escherichia coli. Kuhlman T, Zhang Z, Saier MH Jr, Hwa T. Proc Natl Acad Sci U S A. 2007 Apr 3;104(14):6043-8. doi: 10.1073/pnas.0606717104. Epub 2007 Mar 21. 10.1073/pnas.0606717104 PubMed 17376875