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AncBird+Q111E
(Plasmid #196454)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 196454 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pFastBac Dual
  • Backbone manufacturer
    Thermo Fisher (Invitrogen)
  • Backbone size w/o insert (bp) 5235
  • Total vector size (bp) 9101
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ATP1A1
  • Species
    Ancestral Aves
  • Insert Size (bp)
    3090
  • Promoter PH

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer ATTCCGGATTATTCATACCGTCCCACCATCG
  • 3′ sequencing primer GTGGTATGGCTGATTATGATCCTCTAGTACTTCTC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ATP1B1
  • Species
    Ancestral Aves
  • Insert Size (bp)
    928
  • Promoter p10

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CGGGTTCCTTCCGGTATTGTCTCCTTC
  • 3′ sequencing primer ACGGACCTTTAATTCAACCCAACACAATATA
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    GeneArt Gene Synthesis (Thermo Fisher Scientific)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AncBird+Q111E was a gift from Susanne Dobler (Addgene plasmid # 196454 ; http://n2t.net/addgene:196454 ; RRID:Addgene_196454)

  • For your References section:

    Historical Contingency Shapes Toxin Resistance in a Specialist Avian Predator. Mohammadi S, Pradhan S, Hoffmann FG, Herrera-Álvarez S, Deng Y, Eacock A, Dobler S, Storz JF, Rowland HM. bioRxiv 2025.07.04.662692 10.1101/2025.07.04.662692
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