pPB-TREG3G-PE2-rtTA3G-P2A-eGFP
(Plasmid
#196971)
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PurposePiggybac vector with doxycyclin-inducible prime editor for mammalian cells
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Depositing Lab
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Depositing OrganizationWellcome Sanger Institute
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196971 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 196971-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 196971-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepPB
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameCas9-RT
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Alt nameprime editor
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Insert Size (bp)6321
- Promoter TREG3G
Cloning Information for Gene/Insert 1
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TREG3G-F
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert namertTA3G-P2A-eGFP
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Insert Size (bp)1527
- Promoter PGK
Cloning Information for Gene/Insert 2
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer PGK-F
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 196971-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 196971-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPB-TREG3G-PE2-rtTA3G-P2A-eGFP was a gift from Leopold Parts (Addgene plasmid # 196971 ; http://n2t.net/addgene:196971 ; RRID:Addgene_196971) -
For your References section:
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants. Koeppel J, Weller J, Peets EM, Pallaseni A, Kuzmin I, Raudvere U, Peterson H, Liberante FG, Parts L. Nat Biotechnol. 2023 Feb 16. doi: 10.1038/s41587-023-01678-y. 10.1038/s41587-023-01678-y PubMed 36797492
