Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||19699||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberLow Copy
Gene/Insert nameMerlin I
Alt nameNf2 neurofibromatosis 2
SpeciesM. musculus (mouse)
Insert Size (bp)1800
Entrez GeneNf2 (a.k.a. merlin)
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pXJ40-HA-Merlin I was a gift from Filippo Giancotti (Addgene plasmid # 19699 ; http://n2t.net/addgene:19699 ; RRID:Addgene_19699)
For your References section:Merlin/NF-2 mediates contact inhibition of growth by suppressing recruitment of Rac to the plasma membrane. Okada T, Lopez-Lago M, Giancotti FG. J Cell Biol. 2005 Oct 24. 171(2):361-71. 10.1083/jcb.200503165 PubMed 16247032