yWL229 MCP-GFP-eRF3
(Plasmid
#197053)
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PurposepUBC-NLS-HA-2xMCP-1xGFP-eRF3
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197053 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUbC
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepubc-nls-ha-MCP-GFP-eRF3
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SpeciesH. sapiens (human)
- Promoter ubc
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer ubc
- 3′ sequencing primer WPRE (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
yWL229 MCP-GFP-eRF3 was a gift from Robert Singer (Addgene plasmid # 197053 ; http://n2t.net/addgene:197053 ; RRID:Addgene_197053) -
For your References section:
An improved imaging system that corrects MS2-induced RNA destabilization. Li W, Maekiniemi A, Sato H, Osman C, Singer RH. Nat Methods. 2022 Dec;19(12):1558-1562. doi: 10.1038/s41592-022-01658-1. Epub 2022 Nov 10. 10.1038/s41592-022-01658-1 PubMed 36357695
