pmiRFP680-Actin-C-18
(Plasmid
#197219)
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PurposeExpresses the fusion protein of miRFP680-Actin-C-18 in mammalian cells
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Depositing Lab
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Depositing OrganizationWestlake University
Located in China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 197219 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 197219-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 197219-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepN1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemiRFP680-Actin-C-18
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SpeciesM. musculus (mouse)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.researchsquare.com/article/rs-797586/v1 for preprint.
DNA (Catalog # 197219-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 197219-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmiRFP680-Actin-C-18 was a gift from Kiryl Piatkevich (Addgene plasmid # 197219 ; http://n2t.net/addgene:197219 ; RRID:Addgene_197219) -
For your References section:
Quantitative assessment of near-infrared fluorescent proteins. Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, Koster RW, Wu L, Namikawa K, Piatkevich KD. Nat Methods. 2023 Sep 4. doi: 10.1038/s41592-023-01975-z. 10.1038/s41592-023-01975-z PubMed 37666982
